Dr. Srougi earned her B.S. in Biology from the University of Toledo in 2001, and her Ph.D. in Pharmacology from Case Western Reserve University in 2007. Her graduate work, under the supervision of Dr. David A. Boothman, focused on the cellular mechanisms of a novel cancer chemotherapeutic agent beta-lapachone with an emphasis on the DNA damage response. Following completion of her doctoral studies, she completed a post-doctoral fellowship with Dr. Keith Burridge at the University of North Carolina at Chapel Hill where she studied the regulation of the Rho family of small GTPases after DNA damage. Dr. Srougi joined the Biotechnology Program at North Carolina State University in 2011.
My research interests are to understand how cancer cells acquire resistance to DNA damaging radio- and chemotherapeutic agents. I am particularly interested in uncovering how the Rho family of GTPases modulate the cellular response to genotoxic stress. Elucidating these signaling pathways may have significant therapeutic implications in the current treatment of cancer.
Questions Currently Under Investigation with Undergraduates:
1. How does loss of the DNA damage repair protein ATM affect Rho GTPase function?
2. Do agents that cause different types of DNA damage elicit similar pathways of Rho GTPase activation?
3. How are the guanine nucleotide exchange factors (GEFs; activators of Rho GTPases) regulated after DNA damage?
In addition to my laboratory research, I am also interested in the study of teaching and learning. My pedagogical research includes finding ways to improve student scholarship in the biomedical sciences with a focus on developing student-centered activities and inquiry-based laboratories. One of my recent publications describes a new research-based laboratory course on signal transduction.
Srougi, M.C., Thomas-Swanik, J., Chan, J., Marchant, J., and Carson, S. Making Heads or Tails: Planarian Stem Cells in the Classroom. Journal of Microbiology and Biology Education, in press.
Srougi, M.C., and Carson, S. Inquiry Into Chemotherapy-Induced p53 Activation in Cancer Cells as a Model for Teaching Signal Transduction. (2013) Biochemistry and Molecular Biology Education, DOI: 10.1002/bmb.20741. PMID: 24259336
Srougi, M.C., Miller, H.B., Witherow, D.S., and Carson, S. Assessment of a Novel Group-Centered Testing Schema in an Upper-Level Undergraduate Molecular Biotechnology Course. (2013) Biochemistry and Molecular Biology Education, DOI: 10.1002/bmb.20701. PMID: 23868378
Cao, L.L., Li, LS, Spruell, C., Xiao, L., Chakrabarti, G., Bey, E.A., Reinicke, K.E., Bentle, M.S., Moore, Z., Dong, Y., Vo, P., Kabbani, W., Yang, C.R., Wang, X., Fattah, F, Morales, J.C., Motea, E.A., Bornmann, W.G., Yordy, J., and Boothman, D.A. Tumor-Selective, Futile Redox Cycle-Induced Bystander Effects Elicited by NQO1 Bioactivatable Radiosensitizing Drugs. Antioxid. Redox Signal, in press. PMID: 24512128
Bey, E.A., Reinicke, K.E., Srougi, M.C., et. al. Catalase Abrogates β-Lapachone-Induced PARP-1 Hyperaction-Directed Programmed Necrosis in NQO1-Positive Breast Cancers. (2013) Molecular Cancer Therapeutics, 12(10), 2110-20. PMID: 23883585
Dubash, A.D., Guilluy, C., Srougi, M.C., Boulter, E, Burridge, K., and Garcia-Mata R. The Small GTPase RhoA Localizes to the Nucleus and is Activated by Net1 and DNA Damage Signals. (2011) PLoS One, 6(2), e17380. PMID: 21390328
*Srougi, M.C., and Burridge, K. The Nuclear Guanine Nucleotide Exchange Factors Ect2 and Net1 Regulate RhoB-Mediated Cell Death After DNA Damage. (2011) PLoS One, 6(2), e17108. PMID: 21373644 *paper selected for review by the Faculty of 1000
Srougi, M.C., and Burridge, K. Undressing a Cellular Corset: Septins Exposed. (2009) Nature Cell Biology, 11(1),9-10. PMID: 19122592
Bey, E.A., Bentle, M.S., Reinicke, K.E., Dong, Y., and Boothman, D.A. A Novel NQO1-Mediated and PARP-1-Mediated Cell Death Pathway Induced in Non-Small Cell Lung Cancer Cells by β-Lapachone. (2007) Proceedings of the National Academy of Science USA, 104(28), 11832-11837. PMID: 17609380
Bentle, M.S., Dong, Y., Reinicke, K.E., Bey, E.A., and Boothman, D.A. Non-Homologous End Joining is Essential for Cellular Resistance to the Novel Chemotherapeutic Agent β-Lapachone. (2007) Cancer Research, 67(14), 6936-45. PMID: 17638905
Bentle, M.S., Reinicke, K.E., Bey, E.A., Spitz, D.R., and Boothman, D.A. Calcium-Dependent Modulation of PARP-1 Alters Cellular Metabolism and DNA Repair. (2006) The Journal of Biological Chemistry, 281(44), 33684-33696. PMID: 16920718
Bey, E.A., Wuerzberger-Davis, S.M., Pink, J.J., Yang, C.R., Araki, S., Reinicke, K.E., Bentle, M.S., Dong, Y., Cataldo, E., Criswell, T.C., Wagner, M.W., Li, L., Gao, J., and Boothman, D.A. Mornings with Art, Lessons Learned: Feedback Regulation, Restriction Threshold Biology, and Redundancy Govern Molecular Stress Responses. (2006) The Journal of Cellular Physiology, 209(3), 604-610. PMID: 17001694
Bentle, M.S., Bey, E.A., Dong, Y., Reinicke, K.E., and Boothman, D.A. New Trick for Old Drugs: The Anticarcinogenic Potential of DNA Repair Inhibitors. (2006) The Journal of Molecular Histology, 37(5-7), 203-218. PMID: 16868862
Reinicke, K.E., Bey, E.A., Bentle, M.S., et. al. Development of β-Lapachone Prodrugs for Therapy Against Human Cancers with Elevated NAD(P)H:Quinone Oxidoreductase I Levels. (2005) Clinical Cancer Research, 11(8), 3055-64. PMID: 15837761
When not teaching or working in the lab, Melissa enjoys being outdoors, running, and reading. She also loves to spend her free time with her two favorite boys, Scott and Hunter.