Introduce students to a variety of methods for identifying, quantifying and characterizing proteins by modern mass spectrometric approaches.
Using a cell culture, students will perform a total protein digestion for downstream reverse phase separation using nano ultra high pressure liquid chromatography and quantification with the Thermo Scientific Exploris-480 mass spectrometer. Solution-based and PAGE-based proteomics are explored, along with strategies for Protein Quantification.
Lectures:
From Genomics to Proteomics
Intro to Mass Spectrometry
Quantitative Proteomics Overview
Relative Protein Quantification
Absolute Protein Quantification
–OMICS Research: Contemporary Directions
Labs:
Protein Sample Preparation and Quantification, 1D-SDS-PAGE
In-Gel Digestion of Proteins
Filter-Aided Sample Preparation (FASP) – Protein Digestion
