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BIT 572: Proteomics


  • Introduce students to a variety of methods for identifying, quantifying and characterizing proteins by modern mass spectrometric approaches.
  • Using a cell culture, students will perform a total protein digestion for downstream reverse phase separation using nano ultra high pressure liquid chromatography and quantification with the Thermo Scientific Exploris-480 mass spectrometer. Solution-based and PAGE-based proteomics are explored, along with strategies for Protein Quantification.


  1. From Genomics to Proteomics
  2. Intro to Mass Spectrometry
  3. Quantitative Proteomics Overview
  4. Relative Protein Quantification
  5. Absolute Protein Quantification
  6. –OMICS Research: Contemporary Directions


  1. Protein Sample Preparation and Quantification, 1D-SDS-PAGE
  2. In-Gel Digestion of Proteins
  3. Filter-Aided Sample Preparation (FASP) – Protein Digestion
  4. Phosphoproteomics: Enrichment of Phosphoproteins
  5. Bioinformatic Analysis of Data
  6. SRM/MRM/PRM for Protein Quantification

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