BIT 464/564: Protein Purification
Overview:
- Introduce students to basic skills and theory needed purify recombinant proteins from microbial expression hosts using FPLC
- Use enzymes from hyperthermophiles to simplify assays and minimize stability problems
Labs:
- FPLC Operation – Purification of Protein Standards 1 week
- AEC purification of Thermotoga maritima -gal from Heat-Treated E. coli 1 week
- Purification of Thermotoga maritima -gal from E. coli by AEC, HIC, HAP 3 weeks
- Purification of Thermotoga maritima -gal from E. coli by His-tag/IMEC 1 week
- Finish assays and run gels 1 week