BIT 495/595: Directed Evolution
Overview:
- Participants will be introduced to the theory and applications of directed evolution techniques.
Lecture Topics:
- Introduction to evolution
- Metabolic pathways of E. coli
- Directed evolution in vitro
- Case studies
- Genome sequencing and analysis
Labs:
Project 1: Biosensors
Collaboration with the Williams Lab
- Site directed mutagenesis
- Transformation
- Screening: flow cytometry
- Sequencing analysis
Project 2: Yeast metabolism
Collaboration with the Heil Lab
- Adaptive laboratory evolution
- Whole genome sequencing
- Mutation mapping