BIT 495/595: Directed Evolution


  • Participants will be introduced to the theory and applications of directed evolution techniques.
  • Students will apply directed evolution principles to produce microbes with a specific phenotype.

Lecture topics:

  • Introduction to directed evolution
  • Metabolic pathways of E. coli
  • Directed evolution in vitro
  • Case studies
  • Genome sequencing and analysis


  1. Students work in groups to choose their selective pressure and design their experimental setup.
  2. Students apply experimental scheme and monitor the progression of their phenotype.
  3. Students send out genomic samples for sequencing.
  4. Students analyze genomic data and prepare a report on their findings.

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