BIT 495/595: Directed Evolution

Overview:

  • Participants will be introduced to the theory and applications of directed evolution techniques.

Lecture Topics:

  • Introduction to evolution
  • Metabolic pathways of E. coli
  • Directed evolution in vitro
  • Case studies
  • Genome sequencing and analysis

Labs:

Project 1: Biosensors

Collaboration with the Williams Lab

  • Site directed mutagenesis
  • Transformation
  • Screening: flow cytometry
  • Sequencing analysis

Project 2: Yeast metabolism

Collaboration with the Heil Lab

  • Adaptive laboratory evolution
  • Whole genome sequencing
  • Mutation mapping

Back to Courses