Overview:

• Experience biotechnology research and explore its impact in research and industry.
• Students will gain hands-on experience with modern biotechnolgy techniques and develop technical skills for future laboratory research and industry postions.

Course Objectives:

• CO 1. Design strategies to manipulate DNA to create new proteins with applications in bioremediation, drug discovery, pharmaceuticals, biomedicine, food science, and many other disciplines.
• CO 2. Explain a variety of techniques in the manipulation of recombinant DNA and protein expression.
• CO 3. Create a detailed written record of experimental procedures, results, and conclusions that is reliable and replicable.
• CO 4. Interpret data and controls related to gene cloning, protein expression, a tested hypothesis, and sequence data. 
• CO 5. Troubleshoot molecular biology experiments that do not work.
• CO 6. Reflect on their own thinking and the thinking of others from a variety of disciplines and backgrounds.
• CO 7. Apply critical and creative thinking skills and behaviors in the process of solving problems or addressing questions that span multiple disciplines, including chemistry, biology, pharmacology, physiology, and engineering.
• CO 8. (BIT 510 students only). Design a cloning strategy for manipulating DNA using specialized software with the purpose ofexpressing the recombinant protein in a host system.

Lectures:

Labs:

CaMPARI Cloning

• Hypothesis Design
• Cloning by PCR
• Plasmid DNA Purification/Quantification
• Ligation and Transformation
• Screening Clones (Fluorescence, PCR, Sequencing)
• Gibson Assembly

CaMPARI Expression in E. coli and animal cells

• Hypothesis Testing
• Protein Purification
• SDS-PAGE & Western Blotting
• Animal Cell Culture & Transfection
• Fluorescence Microscopy
• Quantitative Data Anaylsis

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