BIT 410/510: Manipulating Recombinant DNA

Overview:

Experience biotechnology research and explore its impact in research and industry.
Students will gain hands-on experience with modern biotechnolgy techniques and develop technical skills for future laboratory research and industry postions.

CHO GLO cells after receiving 10 uL 100 mM ATP. Lionheart FX was used to capture images. The video is at 2X speed. ATP diffuses from left to right and the fluorescence drops after the ATP reaches the cell. Video and experiment performed by BIT alumni, Rose Krebs.

Lectures:

What is DNA Cloning?
Experimental Design
PCR Cloning and Gibson Assembly
Screening Recombinant Clones

Animal Cell Culture and Aseptic Technique
Next Genertion Squencing
RNA Interference and Genome Editing with CRISPR-Cas9

Labs:

CaMPARI Cloning

Hypothesis Design
Cloning by PCR
Plasmid DNA Purification/Quantification
Ligation and Transformation
Screening Clones (PCR, Sequencing)
Gibson Assembly

CaMPARI Expression in E. coli and animal cells

Hypothesis Testing
Protein Purification
SDS-PAGE and Western Blotting
Animal Cell Culture and Transfection
Fluorescence Microscopy
Quantitative Data Anaylsis

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