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BIT 410/510: Manipulation of Recombinant DNA


  • Experience biotechnology research and explore its impact in research and industry.
  • Students will gain hands-on experience with modern biotechnolgy techniques and develop technical skills for future laboratory research and industry postions.
CHO GLO cells after receiving 10 uL 100 mM ATP. Lionheart FX was used to capture images. The video is at 2X speed. ATP diffuses from left to right and the fluorescence drops after the ATP reaches the cell. Video and experiment performed by BIT alumni, Rose Krebs.


  • Central Concepts in Biotechnology
  • Plasmid Maps & Restriction Enzymes
  • Molecular Cloning Strategies & PCR
  • Gibson Assembly & Transformation
  • Fusion Proteins & Reading Frame
  • Screening Recombinant Clones
  • SDS-PAGE, Antibodies & Western Blot
  • Protein Quantification
  • Animal Cell Culture and Aseptic Technique
  • Cell Signaling & Experimental Design


    CaMPARI Cloning

    • Hypothesis Design
    • Cloning by PCR
    • Plasmid DNA Purification/Quantification
    • Ligation and Transformation
    • Screening Clones (PCR, Sequencing)
    • Gibson Assembly

    CaMPARI Expression in E. coli and animal cells

    • Hypothesis Testing
    • Protein Purification
    • SDS-PAGE and Western Blotting
    • Animal Cell Culture and Transfection
    • Fluorescence Microscopy
    • Quantitative Data Anaylsis

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