BIT 464/564: Protein Purification
Overview
- ntroduce students to basic skills and theory needed purify recombinant proteins from microbial expression hosts using FPLC
- Use enzymes from hyperthermophiles to simplify assays and minimize stability problems
Lectures:
- Introduction to Liquid Chromatography
- Protein Purification Basics
- Liquid Chromatography – Ion Exchange
- Liquid Chromatography – Hydrophobic Interaction
- Liquid Chromatography – Gel Filtration/Affinity
- Protein Folding and Re-folding
- Downstream Processing
Labs:
- FPLC Operation – Purification of Protein Standards -1 week
- AEC purification of Thermotoga maritima -gal from Heat-Treated E. coli -1 week
- Purification of Thermotoga maritima -gal from E. coli by AEC, HIC, HAP -3 weeks
- Purification of Thermotoga maritima -gal from E. coli by His-tag/IMEC -1 week
- Finish assays and run gels -1 week