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Overview:
- To introduce students to the methods and technologies for plant transformation, detection of transgene expression and selection of mutant plants
- To help students develop genetic engineering protocols and design hypotheses that can be tested using plant genetic engineering
Lectures:
- Intro to Plant Genetic Engineering (PGE)
- Agrobacterium Mediated Transformation
- Trait Delivery Systems
- PGE Vector Design
- Exploration of New Genetic Elements for PGE
- Screening and Selection of Considerations and Challenges
- Ethics and Regulation of Engineered Plants
Labs:
- Floral dip, agroinfiltration, microparticle bombardment, and abrasion transformation of plants (Arabidopsis/tobacco)
- Selection and detection of transgenic or genome edited plants (Arabidopsis/tobacco/algae)
- CRISPR knockout of phytoene synthase (algae)
- Formulate a testable hypothesis and design a genetic engineering protocol for a non-model plant
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