BIT 477/577: Metagenomics
We define metagenomics as the use of sequencing-based or molecular biology approaches to discover, explore, and harness the genomic potential of microbial communities in environments. Participants in the BIT 477/577 Metagenomics course have used metagenomics approaches to learn about genetic diversity in numerous environments. They have shared their work on this webpage as part of the course.
- Participants will be introduced to various methods for studying the complex microbial populations surrounding us, including applications, limitations, and health and legal implications.
- Participants will apply high-throughput sequencing techniques to mine the genetic diversity of complex populations, such as the community of microbes growing happily in a kitchen sink.
- Introduction to metagenomics and microbial communities
- Exploiting genome sequencing for drug and biotechnology product discovery
- Approaches and limitations of metagenomic surveys
- Analyzing and visualizing metagenomic data (software and online tools for sequence analysis)
- Isolation of DNA from student-selected microbial community and preparation (Q/C) for high-throughput sequencing with long and short-read sequencing approaches
- Assembly and gene annotation of sequence data from the microbial community using cloud computing
- Use of bioinformatics software for analyses of metagenomic surveys
- Use of cloud-based pipelines for analyzing 16S and whole genome sequences
- Use of software for diversity analyses and hypothesis testing