Skip to main content

BIT 495/595: Experimental Assay Techniques

Throughout this 7.5-week course, we will develop novel Enzyme-Linked Immunosorbent Assays (ELISAs).


The lab activities will guide you through the process of assay development, from quality control checks on materials, to finding the detection range of the assay, to optimizing the assay using Design of Experiments (DOE). During lecture, we will discuss material related to assay design, including primary literature and protocols. Students will share their assay development progress in short presentations and provide peer feedback to one another. You will apply these skills to propose an ELISA against a molecule that you find particularly interesting. Troubleshooting is a core skill you will need for this course- and it can be applied to any research project!

Upon completion of this course, students will be able to:

  • Describe the components of an Enzyme-Linked Immunosorbent Assay (ELISA)
  • Explain their results to peers and supervisors
  • Present their data in small and large groups
  • Troubleshoot throughout the assay development process
  • Document experimental procedures and outcomes
  • Develop a novel Enzyme-Linked Immunosorbent Assay
gloved hand placing a 96-well plate into a plate reader
Gloved hand placing a 96-well plate into a plate reader