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BIT 495/595: Gene Manipulation in Zebrafish


  • Introduce students to the power of genome editing and gene manipulation in Zebrafish
  • Gain hands-on experience designing and implementing experiments to knock out, knock down, and overexpress a gene
BIT 495/595: gene manipulation in zebrafish


  • Introduction to live animal experiments: ethical considerations, development and genetic manipulation
  • Knock-out technologies
  • Knock-down technologies
  • Overexpression techniques
  • Transgenic techniques
  • Phenotype analysis
  • Molecular and biochemical analysis


  • Introduction to Zebrafish molecular techniques
  • Gene knock out using CRISPR/Cas
  • Gene knock down using morpholinos
  • Gene overexpression by mRNA injection
  • Making a GFP transgenic