BIT 495/595: Gene Manipulation in Zebrafish

Overview:

  • Introduce students to the power of genome editing and gene manipulation in Zebrafish
  • Gain hands-on experience designing and implementing experiments to knock out, knock down, and overexpress a gene
BIT 495/595: gene manipulation in zebrafish

Lectures:

  1. Introduction to live animal experiments: ethical considerations, development and genetic manipulation
  2. Knock-out technologies
  3. Knock-down technologies
  4. Overexpression techniques
  5. Transgenic techniques
  6. Phenotype analysis
  7. Molecular and biochemical analysis

Labs:

  1. Introduction to Zebrafish molecular techniques
  2. Gene knock out using CRISPR/Cas
  3. Gene knock down using morpholinos
  4. Gene overexpression by mRNA injection
  5. Making a GFP transgenic