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Overview:
- Introduce students to the power of genome editing and gene manipulation in Zebrafish
- Gain hands-on experience designing and implementing experiments to knock out, knock down, and overexpress a gene
Lectures:
- Introduction to live animal experiments: ethical considerations, development and genetic manipulation
- Knock-out technologies
- Knock-down technologies
- Overexpression techniques
- Transgenic techniques
- Phenotype analysis
- Molecular and biochemical analysis
Labs:
- Introduction to Zebrafish molecular techniques
- Gene knock out using CRISPR/Cas
- Gene knock down using morpholinos
- Gene overexpression by mRNA injection
- Making a GFP transgenic
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