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BIT 495/595 Quantitative Microscopy Image Analysis

Overview

  • Introduction to analyzing the wide array of quantitative data obtainable through microscopy
  • Students will learn how to obtain high-quality microscopy images
  • Students will practice image analysis techniques on different organisms and biological systems
  • Students will also learn cutting edge research from guest lectures, engage in a journal club, create their own publication-quality figures, and create a final project where they pitch their own microscopy and image analysis experiment

Topics:

  • Introduction to Quantitative Microscopy
  • Introduction to Image Analysis
  • Experimental Design
  • Advanced Microscopy Techniques
  • Machine Learning Approaches

Labs:

  • Introduction to Quantitative Microscopy
  • Introduction to Image Analysis
  • AID degradation time course in C. elegans
  • Segmentation and quantification of GFP from AID degradation time course in C. elegans
  • Visualization of MCF-7 breast cancer cells after exposure to beta-lapachone
  • Classifying MCF-7 breast cancer cells using machine learning
  • Visit to the Cellular and Molecular Imaging Facility (CMIF)