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Overview
- To introduce students to a variety of methods for extracting quantitative data from microscopy images
- Students will practice image analysis techniques on a wide range of subjects including different organisms and biological systems
- Students will produce microscopic images suitable for image analysis, assess which features are appropriate to analyze in order to answer a scientific question of interest, and then measure those features with software tools
Topics:
- Microscopy
- Ethics and Pitfalls to Image Acquisition
- What are Images?
- Overview of Image Features
- Introduction to Image Analysis Software
- Fluorescence
- Localization & 3D Imaging
- Timelapses
- Specialized Microscopy Techniques
Labs:
- Basic Microscopy
- Number & Morphology
- Fluorescence Intensity
- Localization
- Timelapses
- Confocal Field Trip