BIT 495/595 – Genome Engineering: CRISPR Technologies

Overview:

• Introduction to how CRISPR-Cas systems work, available tools and the application of these technologies.
• Students will work in teams to design and perform a gene knockout in Serratia marcescens using CRISPR-associated transposons.
• Students will gain experience with CRISPR-equipped bacteriophage.
• Students will communicate broad applications of CRISPR in group presentations as well as novel applications in individual poster presentations.

Class Topics:

• Native CRISPR-Cas Systems
• DNA Repair Mechanisms
• Genetic Engineering with CRISPR
• Available Tools & Applications
• Bioethics of CRISPR

Lab Topics

• Guide RNA Design & Plasmid Mutagenesis
• Golden Gate Assembly
• CRISPR-associated transposons
• CRISPR-equipped bacteriophage
• Experimental Design
• Hypothesis Testing & Data Analysis