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BIT 495/595 – Genome Engineering: CRISPR Technologies


  • Introduction to how CRISPR-Cas systems work, available tools and the application of these technologies.
  • Students will work in teams to design and perform a gene knockout in Serratia marcescens using CRISPR-Cas12a.
  • Students will communicate broad applications of CRISPR in group presentations as well as novel applications in individual poster presentations.

Class Topics:

  • Native CRISPR-Cas Systems
  • DNA Repair Mechanisms & Genetic Engineering
  • Tools & Applications
  • CRISPR-Cas Permutations
  • On and Off-Target Effects
  • CRISPR Bioethics


  • Guide RNA Design & Plasmid Mutagenesis (Weeks 1-3)
  • Golden Gate Assembly (Week 4)
  • Competent Cells & Bacterial Transformation (Weeks 5-6)
  • Experimental Design, Hypothesis Testing & Data Analysis (Weeks 5-8)