BIT 495/595 – Genome Engineering: CRISPR Technologies

Overview:

Introduction to how CRISPR-Cas systems work, available tools and the application of these technologies.
Students will work in teams to design and create a CRISPR-equipped bacteriophage as an alternative antimicrobial against Serratia marcescens.
Students will communicate broad applications of CRISPR in group presentations as well as novel applications in individual poster presentations.

Class Topics:

Native CRISPR-Cas Systems
DNA Repair Mechanisms
Genetic Engineering with CRISPR
Available Tools & Applications
Bioethics of CRISPR

Lab Topics

Guide RNA Design
Site-Directed Mutagenesis
Golden Gate & Gibson Assembly
Lambda Red Recombineering
CRISPR-equipped bacteriophage