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BIT 495/595 – Genome Engineering: CRISPR Technologies


Overview:

  • Introduction to how CRISPR-Cas systems work, available tools and the application of these technologies.
  • Students will work in teams to design and perform a gene knockout in Serratia marcescens using CRISPR-associated transposons.
  • Students will gain experience with CRISPR-equipped bacteriophage.
  • Students will communicate broad applications of CRISPR in group presentations as well as novel applications in individual poster presentations.

Class Topics:

  • Native CRISPR-Cas Systems
  • DNA Repair Mechanisms
  • Genetic Engineering with CRISPR
  • Available Tools & Applications
  • Bioethics of CRISPR

Labs

  • Guide RNA Design & Plasmid Mutagenesis
  • Golden Gate Assembly
  • CRISPR-equipped bacteriophage
  • Experimental Design
  • Hypothesis Testing & Data Analysis