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BIT 495/595 – Protein and Metabolic Engineering


  • Participants will be introduced to the theory and applications of directed evolution techniques.

Lecture Topics:

  • Introduction to evolution
  • Metabolic pathways of E. coli
  • Directed evolution in vitro
  • Case studies
  • Genome sequencing and analysis


Project 1: Biosensors

Collaboration with the Williams Lab

  • Site directed mutagenesis
  • Transformation
  • Screening: flow cytometry
  • Sequencing analysis

Project 2: Yeast metabolism

Collaboration with the Heil Lab

  • Adaptive laboratory evolution
  • Whole genome sequencing
  • Mutation mapping

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