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BIT 495/595: Gene Manipulation in Zebrafish


  • Introduce students to the power of genome editing and gene manipulation in Zebrafish
  • Gain hands-on experience designing and implementing experiments to knock out, knock down, and overexpress a gene
BIT 495/595: gene manipulation in zebrafish


  1. Introduction to live animal experiments: ethical considerations, development and genetic manipulation
  2. Knock-out technologies
  3. Knock-down technologies
  4. Overexpression techniques
  5. Transgenic techniques
  6. Phenotype analysis
  7. Molecular and biochemical analysis


  1. Introduction to Zebrafish molecular techniques
  2. Gene knock out using CRISPR/Cas
  3. Gene knock down using morpholinos
  4. Gene overexpression by mRNA injection
  5. Making a GFP transgenic