BIT 410/510: Manipulating Recombinant DNA

Overview:

  • Experience biotechnology research and explore its impact in research and industry.
  • Students will gain hands-on experience with modern biotechnolgy techniques and develop technical skills for future laboratory research and industry postions.

Lectures:

  1. What is DNA Cloning?
  2. Experimental Design
  3. PCR Cloning and Gibson Assembly
  4. Screening Recombinant Clones
  5. Animal Cell Culture and Aseptic Technique
  6. Next Genertion Squencing
  7. RNA Interference and Genome Editing with CRISPR-Cas9

Labs:

CaMPARI Cloning

  • Hypothesis Design
  • Cloning by PCR
  • Plasmid DNA Purification/Quantification
  • Ligation and Transformation
  • Screening Clones (PCR, Sequencing)
  • Gibson Assembly

CaMPARI Expression in E. coli and animal cells

  • Hypothesis Testing
  • Protein Purification
  • SDS-PAGE and Western Blotting
  • Animal Cell Culture and Transfection
  • Fluorescence Microscopy
  • Quantitative Data Anaylsis

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