BIT 466/566: Animal Cell Culture Techniques

Overview:

  • Acquire the necessary practical skills for the isolation of animals cells for in vitro studies,  maintenance of animal cells in vitro and manipulation of animal cells in vitro.
  • Prepare and use cells for the application of molecular techniques.
Tissue culture flasks in incubator.

Lectures:

  1. Introduction to cell culture 
  2. Design of the cell culture laboratory  
  3. Media preparation and formulations  
  4. Primary cell culture  
  5. Maintaining cells: Contamination and storage  
  6. Eukaryotic gene transfer  
  7. Immunohistochemistry 

Labs:

  1. Safety orientation and basic aseptic technique
    1. 1 week
  2. Methods of media preparation and sterilization
    1. 1 week
  3. Primary cell culture
    1. 2 weeks
  4. Cell preservation and measures of cell viability
    1. 1 week
  5. Eukaryotic gene transfer
    1. 1 week
  6. Immunohistochemistry
    1. 1 week

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