BIT 466/566: Animal Cell Culture Techniques

Overview:

• Acquire the necessary practical skills for the isolation of animals cells for in vitro studies,  maintenance of animal cells in vitro and manipulation of animal cells in vitro.
• Prepare and use cells for the application of molecular techniques.

Lectures:

1. Introduction to cell culture 
2. Design of the cell culture laboratory  
3. Media preparation and formulations  
4. Primary cell culture  
5. Maintaining cells: Contamination and storage  
6. Eukaryotic gene transfer  
7. Immunohistochemistry 

Labs:

1. Safety orientation and basic aseptic technique
   1. 1 week
2. Methods of media preparation and sterilization
   1. 1 week
3. Primary cell culture
   1. 2 weeks
4. Cell preservation and measures of cell viability
   1. 1 week
5. Eukaryotic gene transfer
   1. 1 week
6. Immunohistochemistry
   1. 1 week

Back to Courses