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About
About
Contact Us
Mission Statement
Equipment
News
People
People
Faculty and Instructors
Program Coordination
BIT Graduate Faculty
Internal
Minors & Certificate
Minors & Certificate
Undergraduate Minor Requirements
Graduate Certificate in Molecular Biotechnology Requirements
Graduate Minor Requirements
Courses
Courses
Spring 2025
Fall 2024
Spring 2024
Fall 2023
Spring 2023
Fall 2022
Spring 2022
Fall 2021
Spring 2021
Fall 2020
Spring 2020
Research Training & Fellowships
IPERT
Postdoctoral Training
BIT 466/566: Animal Cell Culture Techniques
Overview:
Acquire the necessary practical skills for the isolation of animals cells for in vitro studies, maintenance of animal cells in vitro and manipulation of animal cells in vitro.
Prepare and use cells for the application of molecular techniques.
Tissue culture flasks in incubator.
Lectures:
Introduction to cell culture
Design of the cell culture laboratory
Media preparation and formulations
Primary cell culture
Maintaining cells: Contamination and storage
Eukaryotic gene transfer
Immunohistochemistry
Labs:
Safety orientation and basic aseptic technique
1 week
Methods of media preparation and sterilization
1 week
Primary cell culture
2 weeks
Cell preservation and measures of cell viability
1 week
Eukaryotic gene transfer
1 week
Immunohistochemistry
1 week
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