BIT 466/566: Animal Cell Culture Techniques
Overview:
- Acquire the necessary practical skills for the isolation of animals cells for in vitro studies, maintenance of animal cells in vitro and manipulation of animal cells in vitro.
- Prepare and use cells for the application of molecular techniques.

Lectures:
- Introduction to cell culture
- Design of the cell culture laboratory
- Media preparation and formulations
- Primary cell culture
- Maintaining cells: Contamination and storage
- Eukaryotic gene transfer
- Immunohistochemistry
Labs:
- Safety orientation and basic aseptic technique
- 1 week
- Methods of media preparation and sterilization
- 1 week
- Primary cell culture
- 2 weeks
- Cell preservation and measures of cell viability
- 1 week
- Eukaryotic gene transfer
- 1 week
- Immunohistochemistry
- 1 week