BIT 479/579: High-throughput Discovery

Overview:

  • Participants will learn modern high-throughput approaches that will prepare them to design, validate, and perform cutting-edge screens and experiments.
Three images. Top left: 96-well plate being held by gloved hand. Top view of wells with blue and red liquids. Top right: multichannel plate pipetting pink media into the first two columns of a 96-well plate. Bottom center: 1536 spots on an agar plate. Most spots show bacterial growth.
What is high-throughput discovery?

Lectures:

  • What is high-throughput discovery science?
  • What makes a good screen?
  • Screens and technology.
  • Chemical libraries and databases.
  • Cutting-edge screening technologies.

Labs:

  • Molecular epidemiology qPCR screen
  • Screen for small molecules that inhibit Delftia acidovorans growth
  • Automated metagenomic library preparation.
  • In silico image anaylsis screen
epMotion 5075 preparing 16S libraries for high-throughput sequencing. Timelapse video by Dr, Carlos C. Goller

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