BIT 410/510 Summer 2024
The BIT 410/510 Manipulation of Recombinant DNA course will be offered with two options: an in-person lab or an online course. For more information, please contact Dr. Emily Cartwright (ecartwr@ncsu.edu).
Overview:
- Experience biotechnology research and explore its impact in research and industry.
- Students will gain hands-on experience with modern biotechnolgy techniques and develop technical skills for future laboratory research and industry postions.
Lectures:
- What is DNA Cloning?
- Experimental Design
- PCR Cloning and Gibson Assembly
- Screening Recombinant Clones
- Animal Cell Culture and Aseptic Technique
- Next Generation Sequencing
- RNA Interference and Genome Editing with CRISPR-Cas9
Labs:
CaMPARI Cloning
- Hypothesis Design
- Cloning by PCR
- Plasmid DNA Purification/Quantification
- Ligation and Transformation
- Screening Clones (PCR, Sequencing)
- Gibson Assembly
CaMPARI Expression in E. coli and animal cells
- Hypothesis Testing
- Protein Purification
- SDS-PAGE and Western Blotting
- Animal Cell Culture and Transfection
- Fluorescence Microscopy
- Quantitative Data Anaylsis