BIT 572: Proteomics

Overview:

  • Introduce students to a variety of methods for identifying, quantifying and characterizing proteins by modern mass spectrometric approaches.
  • Using a cell culture, students will perform a total protein digestion for downstream reverse phase separation using nano ultra high pressure liquid chromatography and quantification with the Thermo Scientific Exploris-480 mass spectrometer. Solution-based and PAGE-based proteomics are explored, along with strategies for Protein Quantification.

Lectures:

  1. From Genomics to Proteomics
  2. Intro to Mass Spectrometry
  3. Quantitative Proteomics Overview
  4. Relative Protein Quantification
  5. Absolute Protein Quantification
  6. –OMICS Research: Contemporary Directions

Labs:

  1. Protein Sample Preparation and Quantification, 1D-SDS-PAGE
  2. In-Gel Digestion of Proteins
  3. Filter-Aided Sample Preparation (FASP) – Protein Digestion
  4. Phosphoproteomics: Enrichment of Phosphoproteins
  5. Bioinformatic Analysis of Data
  6. SRM/MRM/PRM for Protein Quantification

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